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1.
Rev. bras. ciênc. vet ; 27(2): 88-92, abr./jun. 2020. il.
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1378302

ABSTRACT

O objetivo do estudo foi comparar o efeito das técnicas hormonais e de luz artificial nas éguas receptoras de embrião acíclicas avaliando as taxas de gestação aos 14 e 28 dias durante a fase de transição de primavera. Os 48 animais foram distribuídos aleatoriamente nos grupos: controle (CONT, n=16), éguas cíclicas na fase ovulatória; luz artificial (LUZ, n=16), éguas acíclicas submetidas ao tratamento de luz artificial; e hormônio (HORM, n= 16), éguas acíclicas submetidas ao protocolo hormonal na fase de transição. As éguas do grupo LUZ foram estimuladas por 60 dias com luz artificial durante cinco horas por dia. Nos grupos CONT e LUZ, quando observada a presença de folículo ≥ 35 mm de diâmetro e edema uterino ≥ grau II, foram administrados 1,5 mg de acetato de deslorelina e 1500 UI de hCG para induzir a ovulação. As éguas do grupo HORM foram tratadas com três doses de 1,5 mg de benzoato de estradiol e seguiram os mesmos protocolos dos Grupos CONT e LUZ. Foi avaliada a taxa de gestação por ultrassonografia aos 14 dias e confirmação aos 28 em todos os grupos experimentais. Foi realizada análise descritiva e teste Qui-quadrado (significância de 5%). Taxas de gestação aos 14 e 28 dias foram semelhantes (p>0,05) entre todos os grupos. Os tratamentos HORM e LUZ durante o período de transição inverno-primavera mostraram-se eficazes para atender ao programa de transferência de embrião. Por ser um método mais natural, o protocolo LUZ tem potencial como mais uma ferramenta biotecnológica na reprodução de equinos.


The aim of this study was to compare the effect of hormonal and artificial light techniques on acyclic embryo recipient mares by assessing pregnancy rates at 14 and 28 days during the spring transition period. The 48 animals were randomly assigned to the groups: control (CONT, n = 16), cyclic mares in the ovulatory phase; artificial light (LIGHT, n = 16), acyclic mares subjected to artificial light treatment; and hormone (HORM, n = 16), acyclic mares submitted to hormonal protocol in transition phase. In the LIGHT group, mares were stimulated with artificial light for five hours a day, for 60 days. In CONT and LIGHT groups, when a follicle ≥ 35 mm in diameter and uterine edema ≥ grade II were observed, 1.5 mg of deslorelin acetate and 1500 IU hCG were administered to induce ovulation. In the HORM group, mares were treated with three doses of 1.5 mg of estradiol benzoate and followed the same protocols as the CONT and LIGHT groups. Pregnancy rate was assessed by ultrasound at 14 days and confirmation at 28 days in all experimental groups. Descriptive analysis and chi-square test (5% significance) were performed. Pregnancy rates at 14 and 28 days were similar (p> 0.05) among all groups. The HORM and LIGHT treatments during the winter-spring transition period proved to be effective during the embryo transfer programs. As it is a more natural method, the LIGHT protocol has the potential to be one more biotechnological tool in equine reproduction.


Subject(s)
Animals , Ovulation/physiology , Pregnancy, Animal/physiology , Reproductive Techniques, Assisted/veterinary , Embryo, Mammalian , Embryo Transfer/veterinary , Fertility , Horses/embryology , Phototherapy/veterinary , Seasons , Anestrus
2.
Rev. bras. ciênc. vet ; 27(2): 88-92, abr./jun. 2020. graf
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1491670

ABSTRACT

O objetivo do estudo foi comparar o efeito das técnicas hormonais e de luz artificial nas éguas receptoras de embrião acíclicas avaliando as taxas de gestação aos 14 e 28 dias durante a fase de transição de primavera. Os 48 animais foram distribuídos aleatoriamente nos grupos: controle (CONT, n=16), éguas cíclicas na fase ovulatória; luz artificial (LUZ, n=16), éguas acíclicas submetidas ao tratamento de luz artificial; e hormônio (HORM, n= 16), éguas acíclicas submetidas ao protocolo hormonal na fase de transição. As éguas do grupo LUZ foram estimuladas por 60 dias com luz artificial durante cinco horas por dia. Nos grupos CONT e LUZ, quando observada a presença de folículo 35 mm de diâmetro e edema uterino grau II, foram administrados 1,5 mg de acetato de deslorelina e 1500 UI de hCG para induzir a ovulação. As éguas do grupo HORM foram tratadas com três doses de 1,5 mg de benzoato de estradiol e seguiram os mesmos protocolos dos Grupos CONT e LUZ. Foi avaliada a taxa de gestação por ultrassonografia aos 14 dias e confirmação aos 28 em todos os grupos experimentais. Foi realizada análise descritiva e teste Qui-quadrado (significância de 5%). Taxas de gestação aos 14 e 28 dias foram semelhantes (p>0,05) entre todos os grupos. Os tratamentos HORM e LUZ durante o período de transição inverno-primavera mostraram-se eficazes para atender ao programa de transferência de embr


The aim of this study was to compare the effect of hormonal and artificial light techniques on acyclic embryo recipient mares by assessing pregnancy rates at 14 and 28 days during the spring transition period. The 48 animals were randomly assigned to the groups: control (CONT, n = 16), cyclic mares in the ovulatory phase; artificial light (LIGHT, n = 16), acyclic mares subjected to artificial light treatment; and hormone (HORM, n = 16), acyclic mares submitted to hormonal protocol in transition phase. In the LIGHT group, mares were stimulated with artificial light for five hours a day, for 60 days. In CONT and LIGHT groups, when a follicle ≥ 35 mm in diameter and uterine edema ≥ grade II were observed, 1.5 mg of deslorelin acetate and 1500 IU hCG were administered to induce ovulation. In the HORM group, mares were treated with three doses of 1.5 mg of estradiol benzoate and followed the same protocols as the CONT and LIGHT groups. Pregnancy rate was assessed by ultrasound at 14 days and confirmation at 28 days in all experimental groups. Descriptive analysis and chi-square test (5% significance) were performed. Pregnancy rates at 14 and 28 days were similar (p> 0.05) among all groups. The HORM and LIGHT treatments during the winter-spring transition period proved to be effective during the embryo transfer programs. As it is a more natural method, the LIGHT protocol has the potential to be one more biotechnological tool in equine reproduction.


Subject(s)
Female , Animals , Anestrus , Horses/embryology , Horses/physiology , Light , Pregnancy, Animal
3.
Rev. cient. (Maracaibo) ; 16(2): 136-141, mar. 2006. graf
Article in Spanish | LILACS | ID: lil-630944

ABSTRACT

Se determinó la actividad reproductiva de ovejas Pelibuey durante la época de anestro, a 19° latitud norte. Se utilizaron 26 ovejas Pelibuey adultas y 21 ovejas nulíparas, hijas de las anteriores. Durante el primer año las ovejas adultas y nulíparas fueron sometidas diariamente a la detección de celos, de diciembre a julio, utilizando machos Pelibuey provistos de mandil. Durante el segundo año, las hembras fueron aisladas de los machos y se determinó la actividad ovárica por medio de concentraciones de progesterona en los mismos meses. En el primer año, la proporción de estros encontrada de enero a abril fue mayor (P < 0,05) en las ovejas adultas (100; 96,1; 84,6 y 96,1%) que en las nulíparas (42,8; 57,1; 52,3; 61,9%), respectivamente. Diez ovejas adultas y cuatro nulíparas ciclaron de manera regular y continua durante todo el período de observación. Durante el segundo año, la actividad ovulatoria se comportó en forma diferente en ambos grupos; a partir de marzo, se muestra un descenso el cual se mantiene hasta junio en las adultas y en julio en las nulíparas. Además, los porcentajes fueron significativamente diferentes. Ocho ovejas adultas tuvieron actividad ovulatoria continua. Se concluye que algunas ovejas Pelibuey tienen capacidad para mostrar actividad reproductiva continua durante los meses del anestro. Dicha actividad es menor en las hembras nulíparas que en las adultas. La presencia del macho durante la detección de celos ejerce un efecto estimulatorio sobre la actividad estral. La identificación de ovejas con capacidad para ciclar en forma continua, abre la posibilidad de realizar un programa de selección tendente a fijar esta característica.


Reproductive activity of Pelibuey ewes during the anoestrus was determined at 19° lat.N. Twenty six adult Pelibuey ewes and their twenty one nuliparous daughters were used. During the first year, adult and nuliparous ewes were subjected to daily estrous detection from December to July with Pelibuey rams fitted with an apron. During the second year, females were isolated from males and ovarian activity was followed by progesterone concentrations for the same months. Monthly estrous proportion from January to April was higher (P < 0.05) in adult (100, 96.1, 84.6 y 96.1%) than in nuliparous ewes (42.8, 57.1, 52.3, 61.9%). In the first year, ten adult and four nuliparous ewes cycled in a regular and continuous manner during the whole observation period. During the second year, ovulatory activity showed different paterns in the experimental groups; following March, there was a percentage reduction up to June (adults) and July (nuliparous). Eigth adult ewes showed a continuos ovulatory activity. It is concluded that some Pelibuey ewes have the capacity to show a continuous reproductive activity during the anoestrus months. This capacity is lower in nuliparous than in adult ewes. The presence of the male during estrous detection exerts an stimulatory effect on estrous activity. Identification of ewes that cycle in an continuous manner open the possibility to establish a selection program toward this trait.

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